Enhanced arteriogenesis and wound repair in dystrophin-deficient mdx mice

Article

关键词: NITRIC-OXIDE SYNTHASE;    DUCHENNE MUSCULAR-DYSTROPHY;    ENDOTHELIAL GROWTH-FACTOR;    SKELETAL-MUSCLE;    MOLECULAR-BASIS;    SMOOTH-MUSCLE;    EXPRESSION;    REGENERATION;    LACKING;    CELLS;   

DOI  :  10.1161/01.CIR.0000147776.50787.74

来源: SCIE

【 摘 要 】

Background-The absence of functional dystrophin in Duchenne muscular dystrophy (DMD) patients and in mdx mice results in progressive muscle degeneration associated with necrosis, fibrosis, and inflammation. Because vascular supply plays a key role in tissue repair, we examined whether new blood vessel development was altered in mdx mice. Methods and Results-In a model of hindlimb ischemia on femoral artery dissection, hindlimb perfusion, measured by laser Doppler imaging, was higher in mdx mice (0.67+/-0.26) than in wild-type (WT) mice (0.33+/-0.18, P<0.03). In keeping with these data, a significant increase in arteriole length density was found in mdx mice (13.6 +/- 8.4 mm/mm(3)) compared with WT mice (7.8 +/- 4.6 mm/mm(3), P<0.03). Conversely, no difference was observed in capillary density between mice of the 2 genotypes. The enhanced regenerative response was not limited to ischemic skeletal muscle, because in a wound-healing assay, mdx mice showed an accelerated wound closure rate compared with WT mice. Moreover, a vascularization assay in Matrigel plugs containing basic fibroblast growth factor injected subcutaneously revealed an increased length density of arterioles in mdx (46.9+/-14.7 mm/mm(3)) versus WT mice (19.5+/-5.8 mm/mm(3), P<0.001). Finally, serum derived from mdx mice sustained formation of endothelium-derived tubular structures in vitro more efficiently than WT serum. Conclusions-These results demonstrate that arteriogenesis is enhanced in mdx mice both after ischemia and skin wounding and in response to growth factors.

【 授权许可】

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