Helix 8 of leukotriene B-4 receptor 1 inhibits ligand-induced internalization

Article

关键词: PROTEIN-COUPLED RECEPTOR;    AGONIST-INDUCED INTERNALIZATION;    ACTIVATING-FACTOR RECEPTOR;    CRYSTAL-STRUCTURE;    CIRCULAR-DICHROISM;    C-TERMINUS;    PHOSPHORYLATION;    TRAFFICKING;    NMR;    ENDOCYTOSIS;   

DOI  :  10.1096/fj.12-212050

来源: SCIE

【 摘 要 】

Recent crystallographic studies revealed that G-protein-coupled receptors (GPCRs) possess a putative cytoplasmic helical domain, termed helix 8, at the proximal region of the C-terminal tail. However, the significance of helix 8 in GPCR functions and signaling is not fully understood. Helix 8 mutants of leukotriene B-4 receptor type 1 (BLT1) exhibit prolonged activation after ligand stimulation, suggesting some regulatory roles of helix 8 in GPCR signaling. Here, we report the inhibitory role of BLT1 helix 8 on ligand-dependent internalization using BLT1 and platelet- activating factor receptor as model GPCRs. Mutating the dileucine motif in helix 8 of BLT1 to alanines (BLT1 LLAA) enhanced LTB4-dependent internalization of BLT1, whereas wild-type (WT) BLT1 exhibited minimal internalization. Mutational studies revealed that phosphorylation of 5 serine/threonine residues between amino acids 308 and 319 of BLT1 was responsible for enhanced ligand-dependent internalization of BLT1 LLAA. BLT1 LLAA showed enhanced basal and ligand-dependent phosphorylation compared to WT BLT1. Taken together, helix 8 of BLT1 inhibits receptor internalization by suppressing the excessive phosphorylation of the C-terminal tail.-Aratake, Y., Okuno, T., Matsunobu, T., Saeki, K., Takayanagi, R., Furuya, S., Yokomizo, T. Helix 8 of leukotriene B4 receptor 1 inhibits ligand-induced internalization. FASEB J. 26, 4068-4078 (2012). www.fasebj.org

【 授权许可】

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