Mitochondrial BMI1 maintains bioenergetic homeostasis in cells

Article

关键词: HUMAN POLYNUCLEOTIDE PHOSPHORYLASE;    SELF-RENEWAL;    MEDIATED PHOSPHORYLATION;    RNA-POLYMERASE;    CANCER GROWTH;    DNA-DAMAGE;    STEM-CELLS;    STAT3;    TRANSFORMATION;    P53;   

DOI  :  10.1096/fj.201600321R

来源: SCIE

【 摘 要 】

The polycomb complex proto-oncogene BMI1 [B lymphoma Mo-MLV insertion region 1 homolog (mouse)] is essential for self-renewal of normal and cancer stem cells. BMI1-nullmice show severe defects ingrowth, development, and survival. Although BMI1 is known to exert its effect in the nucleus via repression of 2 potent cell-cycle regulators that are encoded by the Ink4a/Arf locus, deletion of this locus only partially rescues BMI1-null phenotypes, which is indicative of alternate mechanisms of action of BMI1. Here, we show that an extranuclear pool of BMI1 localizes to inner mitochondrial membrane and directly regulates mitochondrial RNA (mtRNA) homeostasis and bioenergetics. These mitochondrial functions of BMI1 are independent of its previously described nuclear functions because a nuclear localization-defective mutant BMI1 rescued several bioenergetic defects that we observed in BMI1-depleted cells, for example, mitochondrial respiration, cytochrome c oxidase activity, and ATP production. Mechanistically, BMI1 coprecipitated with polynucleotide phosphorylase, a ribonuclease that is responsible for decay of mtRNA transcripts. Loss of BMI1 enhanced ribonuclease activity of polynucleotide phosphorylase and reduced mtRNA stability. These findings not only establish a novel extranuclear role ofBMI1 in the regulation of mitochondrial bioenergetics, but also provide new mechanistic insights into the role of this protooncogene in stem cell differentiation, neuronal aging, and cancer.

【 授权许可】

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