MicroRNA-5110 regulates pigmentation by cotargeting melanophilin and WNT family member 1

Article

关键词: TRANSCRIPTION FACTOR;    MELANOMA-CELLS;    MYOSIN-VA;    EXPRESSED MICRORNAS;    DOWN-REGULATION;    BINDING DOMAIN;    COAT COLOR;    MELANOCYTES;    PROTEIN;    MITF;   

DOI  :  10.1096/fj.201800040R

来源: SCIE

【 摘 要 】

Mammalian pigmentation requires the production of melanin by melanocytes and its transfer to neighboring keratinocytes. These complex processes are regulated by several molecular pathways. Melanophilin (MLPH) and WNT family member 1 (WNT1), known to be involved in melanin transfer and melanin production, respectively, were predicted to be targets of microRNA-5110 using bioinformatics. In the current study, we investigated the effects of microRNA-5110 on pigmentation in alpaca (Vicugna pacos) melanocytes. In situ hybridization identified high levels of microRNA-5110 in the cytoplasm of alpaca melanocytes. Luciferase activity assays confirmed that MLPH and WNT1 were targeted by microRNA-5110 in these cells. Overexpression and knockdown of microRNA-5110 in alpaca melanocytes downregulated and upregulated MLPH and WNT1 expression at the mRNA and protein levels, respectively. In addition, overexpression and knockdown of microRNA-5110 in alpaca melanocytes decreased and increased, respectively, the mRNA levels of the melanin transfer-related genes, rat sarcoma (RAS)-associated binding (RAB27a) and myosin 5a (MYO5a); the mRNA levels of microphthalmia-associated transcription factor (MITF), tyrosinase (TYR), and tyrosinase-related protein (TYRP)1; and the production of total alkali melanin and pheomelanin. In contrast, overexpression and knockdown of microRNA-5110 increased and decreased the mRNA levels of TYRP2, respectively. Overexpression of microRNA-5110 also increased eumelanin. These results indicate that microRNA-5110 regulates pigmentation in alpaca melanocytes by directly targeting MLPH and WNT1 to affect eumelanin production and transfer.Yang, S., Liu, B., Ji, K., Fan, R., Dong, C. MicroRNA-5110 regulates pigmentation by cotargeting melanophilin and WNT family member 1.

【 授权许可】

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