MAJOR EXPANSION OF CD8+ T-CELLS WITH A PREDOMINANT V-BETA USAGE DURING THE PRIMARY IMMUNE-RESPONSE TO HIV

Article

关键词: IMMUNODEFICIENCY-VIRUS TYPE-1;    VARIABLE REGION GENES;    CD8+ LYMPHOCYTES-T;    VACCINIA VIRUS;    EXPRESSION VECTOR;    INFECTION;    CHAIN;    RECOGNITION;    SEQUENCES;    DIVERSITY;   

DOI  :  10.1038/370463a0

来源: SCIE

【 摘 要 】

A SIGNIFICANT proportion (up to 70%) of individuals experience an acute clinical syndrome of varying severity associated with primary infection with the human immunodeficiency virus (HIV)(1-4). We report here studies on six individuals who showed an acute HIV syndrome which generally resolved within four weeks, concomitant with a dramatic downregulation of viraemia(2-5). To characterize the T-cell-mediated primary immune response to HIV, we used combined semiquantitative polymerase chain reaction assay and cytofluorometry to analyse the T-cell antigen receptor repertoire in sequential peripheral blood mononuclear cells from the patients. We found major oligoclonal expansions in a restricted set of variable-domain beta-chain (V beta) families. Cells expressing the expanded V beta s predominantly expressed the CD8 T-cell differentiation antigen and mediated HIV-specific cytotoxicity. Major oligoclonal expansions of these CD8(+) T lymphocytes may represent an important component of the primary immune response to viral infections and may help to clarify both the immunopathogenic and the protective mechanisms of HIV infection.

【 授权许可】

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