c-kit(+) cells minimally contribute cardiomyocytes to the heart

Article

关键词: CARDIAC STEM-CELLS;    C-KIT;    ADULT;    FUSION;    REGENERATION;    MYOCARDIUM;    EXPRESSION;    MOUSE;    ADOPT;   

DOI  :  10.1038/nature13309

来源: SCIE

【 摘 要 】

If and how the heart regenerates after an injury event is highly debated. c-kit-expressing cardiac progenitor cells have been reported as the primary source for generation of new myocardium after injury. Here we generated two genetic approaches in mice to examine whether endogenous c-kit(+) cells contribute differentiated cardiomyocytes to the heart during development, with ageing or after injury in adulthood. A complementary DNA encoding either Cre recombinase or a tamoxifen-inducible MerCreMer chimaeric protein was targeted to the Kit locus in mice and then bred with reporter lines to permanently mark cell lineage. Endogenous c-kit(+) cells did produce new cardiomyocytes within the heart, although at a percentage of approximately 0.03 or less, and if a preponderance towards cellular fusion is considered, the percentage falls to below approximately 0.008. By contrast, c-kit(+) cells amply generated cardiac endothelial cells. Thus, endogenous c-kit(+) cells cangenerate cardiomyocytes within the heart, although probably at a functionally insignificant level.

【 授权许可】

Free