Topoisomerase II mediates meiotic crossover interference

Article

关键词: SYNAPTONEMAL COMPLEX-FORMATION;    DOUBLE-STRAND BREAKS;    SACCHAROMYCES-CEREVISIAE;    CROSSING-OVER;    CHROMOSOME SYNAPSIS;    MITOTIC CHROMOSOMES;    CHIASMA FORMATION;    YEAST MEIOSIS;    RECOMBINATION;    PROTEIN;   

DOI  :  10.1038/nature13442

来源: SCIE

【 摘 要 】

Spatial patterning is a ubiquitous feature of biological systems. Meiotic crossovers provide an interesting example, defined by the classic phenomenon of crossover interference. Here we identify a molecular pathway for interference by analysing crossover patterns in budding yeast. Topoisomerase II plays a central role, thus identifying a new function for this critical molecule. SUMOylation (of topoisomerase II and axis component Red1) and ubiquitin-mediated removal of SUMOylated proteins are also required. The findings support the hypothesis that crossover interference involves accumulation, relief and redistribution of mechanical stress along the protein/ DNA meshwork of meiotic chromosome axes, with topoisomerase II required to adjust spatial relationships among DNA segments.

【 授权许可】

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