Structural basis for gene regulation by a B-12-dependent photoreceptor

Article

关键词: CRYSTAL-STRUCTURE;    DNA-BINDING;    LIGHT;    EXPRESSION;    FAMILY;    SENSOR;    ADENOSYLCOBALAMIN;    PHOTOLYSIS;    MECHANISM;    B-12;   

DOI  :  10.1038/nature14950

来源: SCIE

【 摘 要 】

Photoreceptor proteins enable organisms to sense and respond to light. The newly discovered CarH-type photoreceptors use a vitamin B-12 derivative, adenosylcobalamin, as the light-sensing chromophore to mediate light-dependent gene regulation. Here we present crystal structures of Thermus thermophilus CarH in all three relevant states: in the dark, both free and bound to operator DNA, and after light exposure. These structures provide visualizations of how adenosylcobalamin mediates CarH tetramer formation in the dark, how this tetramer binds to the promoter 235 element to repress transcription, and how light exposure leads to a large-scale conformational change that activates transcription. In addition to the remarkable functional repurposing of adenosylcobalamin from an enzyme cofactor to a light sensor, we find that nature also repurposed two independent protein modules in assembling CarH. These results expand the biological role of vitamin B-12 and provide fundamental insight into a new mode of light-dependent gene regulation.

【 授权许可】

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