T-cell priming by dendritic cells in lymph nodes occurs in three distinct phases

Article

关键词: IMMUNOLOGICAL SYNAPSE;    ANTIGEN PRESENTATION;    ACTIVATION;    EXPRESSION;    CHEMOKINE;    INDUCTION;   

DOI  :  10.1038/nature02238

来源: SCIE

【 摘 要 】

Primary T-cell responses in lymph nodes (LNs) require contact-dependent information exchange between T cells and dendritic cells (DCs). Because lymphocytes continually enter and leave normal LNs, the resident lymphocyte pool is composed of nonsynchronized cells with different dwell times that display heterogeneous behaviour in mouse LNs in vitro(1-3). Here we employ two-photon microscopy in vivo to study antigen-presenting DCs and naive T cells whose dwell time in LNs was synchronized. During the first 8 h after entering from the blood, T cells underwent multiple short encounters with DCs, progressively decreased their motility, and upregulated activation markers. During the subsequent 12 h T cells formed long-lasting stable conjugates with DCs and began to secrete interleukin-2 and interferon-gamma. On the second day, coinciding with the onset of proliferation, T cells resumed their rapid migration and short DC contacts. Thus, T-cell priming by DCs occurs in three successive stages: transient serial encounters during the first activation phase are followed by a second phase of stable contacts culminating in cytokine production, which makes a transition into a third phase of high motility and rapid proliferation.

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