Spatial profiling of early primate gastrulation in utero

Article

关键词: PLURIPOTENT STEM-CELLS;    LINEAGE ALLOCATION;    GAUSSIAN-PROCESSES;    SELF-ORGANIZATION;    HUMAN EMBRYO;    RNA-SEQ;    NAIVE;    MODEL;    EMBRYOGENESIS;    GENERATION;   

DOI  :  10.1038/s41586-022-04953-1

来源: SCIE

【 摘 要 】

Gastrulation controls the emergence of cellular diversity and axis patterning in the early embryo. In mammals, this transformation is orchestrated by dynamic signalling centres at the interface of embryonic and extraembryonic tissues(1-3). Elucidating the molecular framework of axis formation in vivo is fundamental for our understanding of human development(4-6) and to advance stem-cell-based regenerative approaches'. Here we illuminate early gastrulation of marmoset embryos in utero using spatial transcriptomics and stem-cell-based embryo models. Gaussian process regression based 3D transcriptomes delineate the emergence of the anteriorvisceral endoderm, which is hallmarked by conserved (HHEX, LEFTY2, LHX1) and primate-specific (POSTN, SDC4, FZDS) factors. WNT signalling spatially coordinates the formation of the primitive streak in the embryonic disc and is counteracted by SFRP1 and SFRP2to sustain pluripotency in the anterior domain. Amnion specification occurs at the boundaries ofthe embryonic disc through ID1, ID2 and ID3 in response to BMP signalling, providing a developmental rationale for amnion differentiation of primate pluripotent stem cells (PSCs). Spatial identity mapping demonstrates that primed marmoset PSCs exhibit the highest similarity to the anterior embryonic disc, whereas naive PSCs resemble the preimplantation epiblast. Our 3D transcriptome models reveal the molecular code of lineage specification in the primate embryo and provide an in vivo reference to decipher human development.

【 授权许可】

Free