Central dogma at the single-molecule level in living cells

Review

关键词: REPRESSOR-OPERATOR INTERACTION;    DIFFUSION-DRIVEN MECHANISMS;    STOCHASTIC GENE-EXPRESSION;    ESCHERICHIA-COLI;    RNA-POLYMERASE;    REAL-TIME;    MESSENGER-RNA;    PROTEIN TRANSLOCATION;    FLUORESCENT MOLECULES;    REGULATORY NETWORKS;   

DOI  :  10.1038/nature10315

来源: SCIE

【 摘 要 】

Gene expression originates from individual DNA molecules within living cells. Like many single-molecule processes, gene expression and regulation are stochastic, that is, sporadic in time. This leads to heterogeneity in the messenger-RNA and protein copy numbers in a population of cells with identical genomes. With advanced single-cell fluorescence microscopy, it is now possible to quantify transcriptomes and proteomes with single-molecule sensitivity. Dynamic processes such as transcription-factor binding, transcription and translation can be monitored in real time, providing quantitative descriptions of the central dogma of molecular biology and the demonstration that a stochastic single-molecule event can determine the phenotype of a cell.

【 授权许可】

Free