NINJA connects the co-repressor TOPLESS to jasmonate signalling

Article

关键词: TANDEM AFFINITY PURIFICATION;    FUNCTIONAL GENOMICS;    STRESS-RESPONSE;    PROTEIN FAMILY;    ARABIDOPSIS;    DEGRADATION;    DEFENSE;    GENES;    BIOSYNTHESIS;    METABOLISM;   

DOI  :  10.1038/nature08854

来源: SCIE

【 摘 要 】

Jasmonoyl-isoleucine (JA-Ile) is a plant hormone that regulates a broad array of plant defence and developmental processes(1-5). JA-Ile-responsive gene expression is regulated by the transcriptional activator MYC2 that interacts physically with the jasmonate ZIM-domain (JAZ) repressor proteins. On perception of JA-Ile, JAZ proteins are degraded and JA-Ile-dependent gene expression is activated(6,7). The molecular mechanisms by which JAZ proteins repress gene expression remain unknown. Here we show that the Arabidopsis JAZ proteins recruit the Groucho/Tup1-type corepressor TOPLESS (TPL)(8) and TPL-related proteins (TPRs) through a previously uncharacterized adaptor protein, designated Novel Interactor of JAZ (NINJA). NINJA acts as a transcriptional repressor whose activity is mediated by a functional TPL-binding EAR repression motif. Accordingly, both NINJA and TPL proteins function as negative regulators of jasmonate responses. Our results point to TPL proteins as general co-repressors that affect multiple signalling pathways through the interaction with specific adaptor proteins. This new insight reveals how stress-related and growth-related signalling cascades use common molecular mechanisms to regulate gene expression in plants.

【 授权许可】

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