DNA synthesis provides the driving force to accelerate DNA unwinding by a helicase

Article

关键词: SINGLE-STRANDED-DNA;    REPLICATION FORK;    BACTERIOPHAGE T7;    BINDING PROTEIN;    GENE-4 PROTEIN;    POLYMERASE;    PURIFICATION;    MECHANISMS;    DEFICIENT;   

DOI  :  10.1038/nature03615

来源: SCIE

【 摘 要 】

Helicases are molecular motors that use the energy of nucleoside 5'-triphosphate (NTP) hydrolysis to translocate along a nucleic acid strand and catalyse reactions such as DNA unwinding. The ring-shaped helicase(1) of bacteriophage T7 translocates along single-stranded ( ss) DNA at a speed of 130 bases per second(2); however, T7 helicase slows down nearly tenfold when unwinding the strands of duplex DNA(3). Here, we report that T7 DNA polymerase, which is unable to catalyse strand displacement DNA synthesis by itself, can increase the unwinding rate to 114 base pairs per second, bringing the helicase up to similar speeds compared to its translocation along ssDNA. The helicase rate of stimulation depends upon the DNA synthesis rate and does not rely on specific interactions between T7 DNA polymerase and the carboxy-terminal residues of T7 helicase. Efficient duplex DNA synthesis is achieved only by the combined action of the helicase and polymerase. The strand displacement DNA synthesis by the DNA polymerase depends on the unwinding activity of the helicase, which provides ssDNA template. The rapid trapping of the ssDNA bases by the DNA synthesis activity of the polymerase in turn drives the helicase to move forward through duplex DNA at speeds similar to those observed along ssDNA.

【 授权许可】

Free