Activation of CaMKII in single dendritic spines during long-term potentiation

Article

关键词: PROTEIN-KINASE-II;    YELLOW FLUORESCENT PROTEIN;    RESONANCE ENERGY-TRANSFER;    CA2+ CHANNELS;    SYNAPTIC PLASTICITY;    LTP;    INDUCTION;    NEURONS;    RAS;    PHOSPHORYLATION;   

DOI  :  10.1038/nature07842

来源: SCIE

【 摘 要 】

Calcium/calmodulin-dependent kinase II (CaMKII) plays a central part in long-term potentiation (LTP), which underlies some forms of learning and memory. Here we monitored the spatiotemporal dynamics of CaMKII activation in individual dendritic spines during LTP using two-photon fluorescence lifetime imaging microscopy, in combination with two-photon glutamate uncaging. Induction of LTP and associated spine enlargement in single spines triggered transient (similar to 1 min) CaMKII activation restricted to the stimulated spines. CaMKII in spines was specifically activated by NMDA receptors and L-type voltage-sensitive calcium channels, presumably by nanodomain Ca2+ near the channels, in response to glutamate uncaging and depolarization, respectively. The high degree of compartmentalization and channel specificity of CaMKII signalling allow stimuli-specific spatiotemporal patterns of CaMKII signalling and may be important for synapse-specificity of synaptic plasticity.

【 授权许可】

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