A SUMOylation-dependent pathway mediates transrepression of inflammatory response genes by PPAR-gamma

Article

关键词: ACTIVATED RECEPTOR-GAMMA;    OXIDE SYNTHASE GENE;    MACROPHAGE ACTIVATION;    TRANSCRIPTION FACTORS;    COREPRESSOR COMPLEX;    ANDROGEN-RECEPTOR;    NUCLEAR RECEPTORS;    CO-REPRESSOR;    PROTEINS;    LIGAND;   

DOI  :  10.1038/nature03988

来源: SCIE

【 摘 要 】

Peroxisome proliferator-activated receptor-gamma (PPAR-gamma) has essential roles in adipogenesis and glucose homeostasis, and is a molecular target of insulin-sensitizing drugs(1-3). Although the ability of PPAR-gamma agonists to antagonize inflammatory responses by transrepression of nuclear factor kappa B (NF-kappa B) target genes is linked to antidiabetic(4) and antiatherogenic actions(5), the mechanisms remain poorly understood. Here we report the identification of a molecular pathway by which PPAR-gamma represses the transcriptional activation of inflammatory response genes in mouse macrophages. The initial step of this pathway involves ligand-dependent SUMOylation of the PPAR-gamma ligand-binding domain, which targets PPAR-gamma to nuclear receptor corepressor (NCoR)-histone deacetylase-3 (HDAC3) complexes on inflammatory gene promoters. This in turn prevents recruitment of the ubiquitylation/19S proteosome machinery that normally mediates the signal-dependent removal of corepressor complexes required for gene activation. As a result, NCoR complexes are not cleared from the promoter and target genes are maintained in a repressed state. This mechanism provides an explanation for how an agonist-bound nuclear receptor can be converted from an activator of transcription to a promoter-specific repressor of NF-kappa B target genes that regulate immunity and homeostasis.

【 授权许可】

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