Spatial organization within a niche as a determinant of stem-cell fate

Article

关键词: HAIR FOLLICLE BULGE;    LABEL-RETAINING CELLS;    SELF-RENEWAL;    WOUND REPAIR;    IN-VIVO;    HOMEOSTASIS;    DISTINCT;    SKIN;    LGR5;    IDENTIFICATION;   

DOI  :  10.1038/nature12602

来源: SCIE

【 摘 要 】

Stem-cell niches in mammalian tissues are often heterogeneous and compartmentalized; however, whether distinct niche locations determine different stem-cell fates remains unclear. To test this hypothesis, here we use the mouse hair follicle niche and combine intravital microscopy with genetic lineage tracing to re-visit the same stem-cell lineages, from their exact place of origin, throughout regeneration in live mice. Using this method, we show directly that the position of a stem cell within the hair follicle niche can predict whether it is likely to remain uncommitted, generate precursors or commit to a differentiated fate. Furthermore, using laser ablation we demonstrate that hair follicle stem cells are dispensable for regeneration, and that epithelial cells, which do not normally participate in hair growth, re-populate the lost stem-cell compartment and sustain hair regeneration. This study provides a general model for niche-induced fate determination in adult tissues.

【 授权许可】

Free