REQUIREMENT OF AN ICE/CED-3 PROTEASE FOR FAS/APO-1-MEDIATED APOPTOSIS

Article

关键词: TUMOR-NECROSIS-FACTOR;    CELL-SURFACE ANTIGEN;    INTERLEUKIN-1-BETA CONVERTING ENZYME;    DEATH GENE CED-3;    MOLECULAR-CLONING;    MONOCLONAL-ANTIBODY;    VIRUS ENCODES;    FAS;    INDUCTION;    RECEPTOR;   

DOI  :  10.1038/375081a0

来源: SCIE

【 摘 要 】

THE Fas/APO-1 receptor is one of the major regulators of apoptosis(1-7). We report here that Fas/APO-1-mediated apoptosis requires the activation of a new class of cysteine proteases, including interleukin-1 beta-converting enzyme (ICE)(8-10) which are homologous to the product of the Caenorhabditis elegans cell-death gene ced-3 (refs 11, 12). Triggering of Fas/APO-1 rapidly stimulated the proteolytic activity of ICE. Overexpression of ICE, achieved by electroporation and microinjection, strongly potentiated Fas/APO-1-mediated cell death. In addition, inhibition of ICE activity by protease inhibitors, as well as by transient expression of the pox virus-derived serpin inhibitor CrmA or an antisense ICE construct, substantially suppressed Fas/APO-1-triggered cell death. We conclude that activation of ICE or an ICE-related protease is a critical event in Fas/APO-1-mediated cell death.

【 授权许可】

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