A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish

Article

关键词: TUMOR-SUPPRESSOR PTEN;    ENDOTHELIAL-CELLS;    NOX INHIBITOR;    DUAL OXIDASES;    INFLAMMATION;    CHEMOTAXIS;    MIGRATION;    GROWTH;    NEUTROPHILS;    EXPRESSION;   

DOI  :  10.1038/nature08119

来源: SCIE

【 摘 要 】

Barrier structures (for example, epithelia around tissues and plasma membranes around cells) are required for internal homeostasis and protection from pathogens. Wound detection and healing represent a dormant morphogenetic program that can be rapidly executed to restore barrier integrity and tissue homeostasis. In animals, initial steps include recruitment of leukocytes to the site of injury across distances of hundreds of micrometres within minutes of wounding. The spatial signals that direct this immediate tissue response are unknown. Owing to their fast diffusion and versatile biological activities, reactive oxygen species, including hydrogen peroxide (H2O2), are interesting candidates for wound-to-leukocyte signalling. Here we probe the role of H2O2 during the early events of wound responses in zebrafish larvae expressing a genetically encoded H2O2 sensor(1). This reporter revealed a sustained rise in H2O2 concentration at the wound margin, starting similar to 3 min after wounding and peaking at similar to 20 min, which extended similar to 100-200 mu m into the tail-fin epithelium as a decreasing concentration gradient. Using pharmacological and genetic inhibition, we show that this gradient is created by dual oxidase (Duox), and that it is required for rapid recruitment of leukocytes to the wound. This is the first observation, to our knowledge, of a tissue-scale H2O2 pattern, and the first evidence that H2O2 signals to leukocytes in tissues, in addition to its known antiseptic role.

【 授权许可】

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