HRG-9 homologues regulate haem trafficking from haem-enriched compartments

Article

关键词: METABOLIC CRISES;    RNA-SEQ;    ZEBRAFISH;    RESISTANCE;    RECEPTOR;    HOMEOSTASIS;    DYNAMICS;    EMBRYO;    IRON;   

DOI  :  10.1038/s41586-022-05347-z

来源: SCIE

【 摘 要 】

Haem is an iron-containing tetrapyrrole that is critical for a variety of cellular and physiological processes(1-3). Haem binding proteins are present in almost all cellular compartments, but the molecular mechanismsthat regulate the transport and use of haem within the cell remain poorly understood(2,3). Here we show that haem-responsive gene 9 (HRG-9) (also known as transport and Golgi organization 2 (TANGO2)) is an evolutionarily conserved haem chaperone with a crucial role in trafficking haem out of haem storage or synthesis sites in eukaryotic cells. Loss of Caenorhabdids elegans hrg-9 and its paraloguehrg-10 results in the accumulation of haem in lysosome-related organelles, the haem storage site in worms. Similarly, deletion ofthe hrg-9 homologue TANGO2 in yeast and mammalian cells induces haem overload in mitochondria, the site of haem synthesis. We demonstrate that TANGO2 binds haem and transfers it from cellular membranesto apo-haemoproteins. Notably, homozygous tango2(-/-) zebrafish larvae develop pleiotropic symptoms including encephalopathy, cardiac arrhythmia and myopathy, and die during early development. These defects partially resemble the symptoms of human TANG02-related metabolic encephalopathy and arrhythmias, a hereditary disease caused by mutations in TANGO2(4-8). Thus, the identification of HRG-9 as an intracellular haem chaperone provides a biological basis for exploring the aetiology and treatment of TANGO2-related disorders.

【 授权许可】

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