Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome

Article

关键词: FAMILIAL MEDITERRANEAN FEVER;    GTP-BINDING PROTEINS;    CLOSTRIDIUM-DIFFICILE;    BURKHOLDERIA-CENOCEPACIA;    TOXIN-B;    ACTIN CYTOSKELETON;    VIBRIO-CHOLERAE;    RTX TOXIN;    ACTIVATION;    SYSTEM;   

DOI  :  10.1038/nature13449

来源: SCIE

【 摘 要 】

Cytosolic inflammasome complexes mediated by a pattern recognition receptor (PRR) defend against pathogen infection by activating caspase 1. Pyrin, a candidate PRR, can bind to the inflammasome adaptor ASC to form a caspase 1-activating complex(1,2). Mutations in the Pyrin-encoding gene, MEFV, cause a human autoinflammatory disease known as familial Mediterranean fever(3-5). Despite important roles in immunity and disease, the physiological function of Pyrin remains unknown. Here we show that Pyrin mediates caspase 1 inflammasome activation in response to Rho-glucosylation activity of cytotoxin TcdB(6-8), a major virulence factor of Clostridium difficile, which causes most cases of nosocomial diarrhoea. The glucosyltransferase-inactive TcdB mutant loses the inflammasome-stimulating activity. Other Rho-inactivating toxins, including FIC-domain adenylyltransferases (Vibrioparahaemolyticus VopS and Histophilus somnilbpA) and Clostridium botulinum ADP-ribosylating C3 toxin, can also biochemically activate the Pyrin inflammasome in their enzymatic activitydependent manner. These toxins all target the Rho subfamily and modify a switch-I residue. We further demonstrate that Burkholderia cenocepacia inactivates RHOA by deamidating Asn 41, also in the switch-I region, and thereby triggers Pyrin inflammasome activation, both of which require the bacterial type VI secretion system (T6SS). Loss of the Pyrin inflammasome causes elevated intra-macrophage growth of B. cenocepacia and diminished lung inflammation in mice. Thus, Pyrin functions to sense pathogen modification and inactivation of Rho GTPases, representing a new paradigm in mammalian innate immunity.

【 授权许可】

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