eIF3 targets cell-proliferation messenger RNAs for translational activation or repression

Article

关键词: C-JUN;    PREINITIATION COMPLEX;    VIRAL TRANSLATION;    BINDING PROTEIN;    PAR-CLIP;    INITIATION;    SITES;    IDENTIFICATION;    TRANSFORMATION;    SPECIFICITY;   

DOI  :  10.1038/nature14267

来源: SCIE

【 摘 要 】

Regulation of protein synthesis is fundamental for all aspects of eukaryotic biology by controlling development, homeostasis and stress responses(1,2). The 13-subunit, 800-kilodalton eukaryotic initiation factor 3 (eIF3) organizes initiation factor and ribosome interactions required for productive translation(3). However, current understanding of eIF3 function does not explain genetic evidence correlating eIF3 deregulation with tissue-specific cancers and developmental defects(4). Here we report the genome-wide discovery of human transcripts that interact with eIF3 using photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation (PAR-CLIP)(5). eIF3 binds to a highly specific program of messenger RNAs involved in cell growth control processes, including cell cycling, differentiation and apoptosis, via them RNA 5' untranslated region. Surprisingly, functional analysis of the interaction between eIF3 and two mRNAs encoding the cell proliferation regulators c-JUN and BTG1 reveals that eIF3 uses different modes of RNA stem-loop binding to exert either translational activation or repression. Our findings illuminate a new role for eIF3 in governing a specialized repertoire of gene expression and suggest that binding of eIF3 to specific mRNAs could be targeted to control carcinogenesis.

【 授权许可】

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