Ultrafast structural changes within a photosynthetic reaction centre | |
Article | |
关键词: INITIAL CHARGE SEPARATION; ELECTRON-TRANSFER; RHODOPSEUDOMONAS-VIRIDIS; MEMBRANE-PROTEIN; HIGH-THROUGHPUT; SPECIAL PAIR; FORCE-FIELD; CRYSTALLOGRAPHY; SPECTROSCOPY; MECHANISM; | |
DOI : 10.1038/s41586-020-3000-7 | |
来源: SCIE |
【 摘 要 】
Photosynthetic reaction centres harvest the energy content of sunlight by transporting electrons across an energy-transducing biological membrane. Here we use time-resolved serial femtosecond crystallography(1) using an X-ray free-electron laser(2) to observe light-induced structural changes in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds. Structural perturbations first occur at the special pair of chlorophyll molecules of the photosynthetic reaction centre that are photo-oxidized by light. Electron transfer to the menaquinone acceptor on the opposite side of the membrane induces a movement of this cofactor together with lower amplitude protein rearrangements. These observations reveal how proteins use conformational dynamics to stabilize the charge-separation steps of electron-transfer reactions. Time-resolved serial femtosecond crystallography is used to reveal the structural changes that stabilize the charge-separation steps of electron-transfer reactions in the photosynthetic reaction centre of Blastochloris viridis on a timescale of picoseconds.
【 授权许可】
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