Affinity maturation without germinal centres in lymphotoxin-alpha-deficient mice

Article

关键词: FOLLICULAR DENDRITIC CELLS;    B-CELL;    IMMUNE-RESPONSE;    SOMATIC HYPERMUTATION;    PRIMARY IMMUNIZATION;    COMPLEMENT RECEPTOR;    ANTIBODY MUTANTS;    LYMPHOCYTES-B;    T-CELL;    CENTERS;   

DOI  :  10.1038/382462a0

来源: SCIE

【 摘 要 】

AFFINITY maturation by somatic hypermutation is thought to occur within germinal centres(1-4). Mice deficient in lymphotoxin-alpha (LT alpha(-/-) mice) have no lymph nodes or Peyer's patches(5,6), and fail to form germinal centres in the spleen(7). We tested whether germinal centres are essential for maturation of antibody responses to T-cell-dependent antigens. LT alpha(-/-) mice immunized with low doses of (4-hydroxy-3-nitrophenyl)acetyl-ovalbumin (NP-OVA) showed dramatically impaired production of high-affinity anti-NP IgG1. However, LT alpha(-/-) mice immunized with high doses of NP-OVA, even though they failed to produce germinal centres, manifested a high-affinity anti-NP IgG1 response similar to wild-type mice. Furthermore, when LT alpha(-/-) mice were multiply immunized with high doses of NP-OVA, the predominantly expressed anti-NP Vu gene segment VH186.2 showed somatic mutations typical of affinity maturation(8). Thus, B-cell memory and affinity maturation are not absolutely dependent on the presence of germinal centres.

【 授权许可】

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