Transcriptional regulation of autophagy by an FXR-CREB axis

Article

关键词: FARNESOID X RECEPTOR;    COACTIVATOR CRTC2;    LIPID-METABOLISM;    GENOMIC ANALYSIS;    ER STRESS;    PHOSPHORYLATION;    DISEASE;    BINDING;    OBESITY;    LIVER;   

DOI  :  10.1038/nature13949

来源: SCIE

【 摘 要 】

Lysosomal degradation of cytoplasmic components by autophagy is essential for cellular survival and homeostasis under nutrient-deprived conditions(1-4). Acute regulation of autophagy by nutrient-sensing kinases is well defined(3,5-7), but longer-term transcriptional regulation is relatively unknown. Here we show that the fed-state sensing nuclear receptor farnesoid X receptor (FXR)(8,9) and the fasting transcriptional activator cAMP response element-binding protein (CREB)(10,11) coordinately regulate the hepatic autophagy gene network. Pharmacological activation of FXR repressed many autophagy genes and inhibited autophagy even in fasted mice, and feeding-mediated inhibition of macroautophagy was attenuated in FXR-knockout mice. From mouse liver chromatin immunoprecipitation and high-throughput sequencing data(12,15), FXR and CREB binding peaks were detected at 178 and 112 genes, respectively, out of 230 autophagy-related genes, and 78 genes showed shared binding, mostly in their promoter regions. CREB promoted autophagic degradation of lipids, or lipophagy(16), under nutrient-deprived conditions, and FXR inhibited this response. Mechanistically, CREB upregulated autophagy genes, including A tg7, Ulkl and Tfeb, by recruiting the coactivator CRTC2. After feeding or pharmacological activation, FXR trans-repressed these genes by disrupting the functional CREB-CRTC2 complex. This study identifies the new FXR-CREB axis as a key physiological switch regulating autophagy, resulting in sustained nutrient regulation of autophagy during feeding/fasting cycles.

【 授权许可】

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