Homeostatic control of metabolic and functional fitness of Treg cells by LKB1 signalling

Article

关键词: REGULATORY T-CELLS;    ACTIVATION;    RESPONSES;    PD-1;    BETA;    OX40;    DIFFERENTIATION;    COSTIMULATION;    INFLAMMATION;    SURVIVAL;   

DOI  :  10.1038/nature23665

来源: SCIE

【 摘 要 】

Regulatory T cells (T-reg cells) have a pivotal role in the establishment and maintenance of immunological self-tolerance and homeostasis(1,2). Transcriptional programming of regulatory mechanisms facilitates the functional activation of T-reg cells in the prevention of diverse types of inflammatory responses(3,4). It remains unclear how T-reg cells orchestrate their homeostasis and interplay with environmental signals. Here we show that liver kinase B1 (LKB1) programs the metabolic and functional fitness of T-reg cells in the control of immune tolerance and homeostasis. Mice with a T-reg-specific deletion of LKB1 developed a fatal inflammatory disease characterized by excessive T(H)2-type-dominant responses. LKB1 deficiency disrupted T-reg cell survival and mitochondrial fitness and metabolism, but also induced aberrant expression of immune regulatory molecules including the negative co-receptor PD-1 and the TNF receptor superfamily proteins GITR and OX40. Unexpectedly, LKB1 function in T-reg cells was independent of conventional AMPK signalling or the mTORC1-HIF-1 alpha axis, but contributed to the activation of beta-catenin signalling for the control of PD-1 and TNF receptor proteins. Blockade of PD-1 activity reinvigorated the ability of LKB1-deficient T-reg cells to suppress T(H)2 responses and the interplay with dendritic cells primed by thymic stromal lymphopoietin. Thus, T-reg cells use LKB1 signalling to coordinate their metabolic and immunological homeostasis and to prevent apoptotic and functional exhaustion, thereby orchestrating the balance between immunity and tolerance.

【 授权许可】

Free