DNA-binding properties of the yeast SWI/SNF complex

Article

关键词: TRANSCRIPTIONAL ACTIVATOR;    CRUCIFORM DNA;    PROTEIN;    GENES;    ENHANCERS;    HMG1;   

DOI  :  10.1038/379844a0

来源: SCIE

【 摘 要 】

The SWI/SNF complex is required for the enhancement of transcription by many transcriptional activators in yeast(1,2) Genetic and biochemical studies indicate that the complex facilitates activator function by antagonizing chromatin-mediated transcriptional repression(3-6). The absence of known DNA-binding motifs in several SWI/SNF subunits and the failure to identify SWI/SNF-dependent DNA-binding activities in crude yeast extracts have led to the belief that the complex does not bind DNA(7,8). Here we show that the SWI/SNF complex has a high affinity for DNA and that its DNA-binding properties are similar to those of proteins containing HMG-box domains(9). The complex interacts with the minor groove of the DNA helix, binds synthetic four-way junction DNA, and introduces positive supercoils into relaxed plasmid DNA. These properties are likely to be important in the remodelling of chromatin structure by the SWI/SNF complex.

【 授权许可】

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