The replisome uses mRNA as a primer after colliding with RNA polymerase

Article

关键词: STALLED REPLICATION FORKS;    DISCONTINUOUS DNA-REPLICATION;    ESCHERICHIA-COLI;    LAGGING-STRAND;    LEADING-STRAND;    CHAIN GROWTH;    TRANSCRIPTION;    REPAIR;    ORGANIZATION;    ELONGATION;   

DOI  :  10.1038/nature07527

来源: SCIE

【 摘 要 】

Replication forks are impeded by DNA damage and protein - nucleic acid complexes such as transcribing RNA polymerase. For example, head- on collision of the replisome with RNA polymerase results in replication fork arrest. However, co- directional collision of the replisome with RNA polymerase has little or no effect on fork progression. Here we examine co- directional collisions between a replisome and RNA polymerase in vitro. We show that the Escherichia coli replisome uses the RNAtranscript as a primer to continue leading- strand synthesis after the collision with RNA polymerase that is displaced from the DNA. This action results in a discontinuity in the leading strand, yet the replisome remains intact and bound to DNA during the entire process. These findings underscore the notable plasticity by which the replisome operates to circumvent obstacles in its path and may explain why the leading strand is synthesized discontinuously in vivo.

【 授权许可】

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