Signature of long-lived memory CD8(+) T cells in acute SARS-CoV-2 infection

Article

关键词: DIFFERENTIATION;    PROLIFERATION;    EFFECTOR;    SUBSETS;   

DOI  :  10.1038/s41586-021-04280-x

来源: SCIE

【 摘 要 】

Immunological memory is a hallmark of adaptive immunity and facilitates an accelerated and enhanced immune response upon re-infection with the same pathogen(1,2). Since the outbreak of the ongoing COVID-19 pandemic, a key question has focused on which SARS-CoV-2-specific T cells stimulated during acute infection give rise to long-lived memory T cells(3). Here, using spectral flow cytometry combined with cellular indexing of transcriptomes and T cell receptor sequencing, we longitudinally characterized individual SARS-CoV-2-specific CD8(+) T cells of patients with COVID-19 from acute infection to 1 year into recovery and found a distinct signatue identifying long-lived memory CD8(+) T cells. SARS-CoV-2-specific memory CD8(+3) T cells persisting 1 year after acute infection express CD45RA, IL-7 receptor-a and T cell factor 1, but they maintain low expression of CCR7, thus resembling CD45RA(+) effector memory T cells. Tracking individual clones of SARS-CoV-2-specific CD8(+) T cells, we reveal that an interferon signature marks clones that give rise to long-lived cells, whereas prolonged proliferation and mechanistic target of rapamycin signalling are associated with clonal disappearance from the blood. Collectively, we describe a transcriptional signature that marks long-lived, circulating human memory CD8(+) T cells following an acute viral infection.

【 授权许可】

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