Sex-specific chromatin remodelling safeguards transcription in germ cells

Article

关键词: DNA METHYLATION;    RETINOIC ACID;    CPG ISLANDS;    DYNAMICS;    COMPLEX;    FATE;    RECRUITMENT;    GENERATION;    ELEMENTS;    PACKAGE;   

DOI  :  10.1038/s41586-021-04208-5

来源: SCIE

【 摘 要 】

Stability of the epigenetic landscape underpins maintenance of the cell-type-specific transcriptional profile. As one of the main repressive epigenetic systems, DNA methylation has been shown to be important for long-term gene silencing; its loss leads to ectopic and aberrant transcription in differentiated cells and cancer(1). The developing mouse germ line enduresglobal changes in DNA methylation in the absence of widespread transcriptional activation. Here, using an ultra-low-input native chromatin immunoprecipitation approach, we show that following DNA demethylation the gonadal primordial germ cells undergo remodelling of repressive histone modifications, resulting in a sex-specific signature in mice. We further demonstrate that Polycomb has a central role in transcriptional control in the newly hypomethylated germline genome as the genetic loss of Ezh2 leads to aberrant transcriptional activation, retrotransposon derepression and dramatic loss of developing female germ cells. This sex-specific effect of Ezh2 deletion is explained by the distinct landscape of repressive modifications observed in male and female germ cells. Overall, our study provides insight into the dynamic interplay between repressive chromatin modifications in the context of a developmental reprogramming system.

【 授权许可】

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