Calcium triggers exit from meiosis II by targeting the APC/C inhibitor XErp1 for degradation

Article

关键词: CYTOSTATIC FACTOR ARREST;    XENOPUS EGG EXTRACTS;    PROTEIN KINASE-II;    METAPHASE;    DESTRUCTION;    UBIQUITIN;    ANAPHASE;    COMPLEX;    FERTILIZATION;    BINDING;   

DOI  :  10.1038/nature04093

来源: SCIE

【 摘 要 】

Vertebrate eggs awaiting fertilization are arrested at metaphase of meiosis II by a biochemical activity termed cytostatic factor (CSF)(1,2). This activity inhibits the anaphase-promoting complex/ cyclosome (APC/C), a ubiquitin ligase that triggers anaphase onset and mitotic/meiotic exit by targeting securin and M-phase cyclins for destruction(3,4,5). On fertilization a transient rise in free intracellular calcium(6) causes release from CSF arrest and thus APC/C activation. Although it has previously been shown that calcium induces the release of APC/C from CSF inhibition through calmodulin-dependent protein kinase II ( CaMKII)(7,8), the relevant substrates of this kinase have not been identified. Recently, we characterized XErp1 (Emi2), an inhibitor of the APC/C and key component of CSF activity in Xenopus egg extract(9). Here we show that calcium-activated CaMKII triggers exit from meiosis II by sensitizing the APC/C inhibitor XErp1 for polo-like kinase 1 (Plx1)-dependent degradation. Phosphorylation of XErp1 by CaMKII leads to the recruitment of Plx1 that in turn triggers the destruction of XErp1 by phosphorylating a site known to serve as a phosphorylation-dependent degradation signal. These results provide a molecular explanation for how the fertilization-induced calcium increase triggers exit from meiosis II.

【 授权许可】

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