Dynamics of nucleosome remodelling by individual ACF complexes

Article

关键词: DNA TRANSLOCATION;    SINGLE-MOLECULE;    HISTONE OCTAMER;    ENERGY-TRANSFER;    CORE PARTICLE;    CHROMATIN;    ISWI;    SWI/SNF;    MECHANISM;    CATALYSIS;   

DOI  :  10.1038/nature08627

来源: SCIE

【 摘 要 】

The ATP-dependent chromatin assembly and remodelling factor (ACF) functions to generate regularly spaced nucleosomes, which are required for heritable gene silencing. The mechanism by which ACF mobilizes nucleosomes remains poorly understood. Here we report a single-molecule FRET study that monitors the remodelling of individual nucleosomes by ACF in real time, revealing previously unknown remodelling intermediates and dynamics. In the presence of ACF and ATP, the nucleosomes exhibit gradual translocation along DNA interrupted by well-defined kinetic pauses that occurred after approximately seven or three to four base pairs of translocation. The binding of ACF, translocation of DNA and exiting of translocation pauses are all ATP-dependent, revealing three distinct functional roles of ATP during remodelling. At equilibrium, a continuously bound ACF complex can move the nucleosome back-and-forth many times before dissociation, indicating that ACF is a highly processive and bidirectional nucleosome translocase.

【 授权许可】

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