High-resolution Xist binding maps reveal two-step spreading during X-chromosome inactivation

Article

关键词: FACULTATIVE HETEROCHROMATIN;    RNA;    GENE;    CHROMATIN;    LOCALIZATION;    ANNOTATION;    DOMAINS;    DESIGN;   

DOI  :  10.1038/nature12719

来源: SCIE

【 摘 要 】

TheXist long noncodingRNA(lncRNA) is essential for X-chromosome inactivation (XCI), the process by which mammals compensate for unequal numbers of sex chromosomes(1-3). During XCI, Xist coats the future inactiveXchromosome(Xi)(4) and recruitsPolycomb repressive complex 2 (PRC2) to the X-inactivation centre (Xic)(5). How Xist spreads silencing on a 150-megabases scale is unclear. Here we generate high-resolution maps of Xist binding on the X chromosome across a developmental time course using CHART-seq. In female cells undergoing XCI de novo, Xist follows a two-step mechanism, initially targeting gene-rich islands before spreading to intervening gene-poor domains. Xist is depleted from genes that escape XCI but may concentrate near escapee boundaries. Xist binding is linearly proportional to PRC2 density and H3 lysine 27 trimethylation (H3K27me3), indicating co-migration of Xist and PRC2. Interestingly, when Xist is acutely stripped off from the Xi in post-XCI cells, Xist recovers quickly within both gene-rich and gene-poor domains on a timescale of hours instead of days, indicating a previously primed Xi chromatin state. We conclude that Xist spreading takes distinct stage-specific forms. During initial establishment, Xist follows a two-step mechanism, but during maintenance, Xist spreads rapidly to both gene-rich and gene-poor regions.

【 授权许可】

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