Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex

Article

关键词: PROTEIN-COUPLED RECEPTORS;    HUMAN-CELLS;    RAF INHIBITION;    TARGET DNA;    RNA;    RESISTANCE;    CANCER;    MELANOMA;    CAS9;    PATHWAY;   

DOI  :  10.1038/nature14136

来源: SCIE

【 摘 要 】

Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. Here we describe structure-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci. We used these engineered Cas9 activation complexes to investigate single-guide RNA (sgRNA) targeting rules for effective transcriptional activation, to demonstrate multiplexed activation of ten genes simultaneously, and to upregulate long intergenic non-coding RNA (lincRNA) transcripts. We also synthesized a library consisting of 70,290 guides targeting all human RefSeq coding isoforms to screen for genes that, upon activation, confer resistance to a BRAF inhibitor. The top hits included genes previously shown to be able to confer resistance, and novel candidates were validated using individual sgRNA and complementary DNA overexpression. A gene expression signature based on the top screening hits correlated with markers of BRAF inhibitor resistance in cell lines and patient-derived samples. These results collectively demonstrate the potential of Cas9-based activators as a powerful genetic perturbation technology.

【 授权许可】

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