Tracing oncogene-driven remodelling of the intestinal stem cell niche

Article

关键词: MICROENVIRONMENTAL REGULATION;    FIELD CANCERIZATION;    TUMOR PROGRESSION;    HOMEOSTASIS;    COMPETITION;    CANCER;    DIFFERENTIATION;    EPITHELIUM;    DYNAMICS;    SIGNALS;   

DOI  :  10.1038/s41586-021-03605-0

来源: SCIE

【 摘 要 】

Interactions between tumour cells and the surrounding microenvironment contribute to tumour progression, metastasis and recurrence(1-3). Although mosaic analyses in Drosophila have advanced our understanding of such interactions(4,5), it has been difficult to engineer parallel approaches in vertebrates. Here we present an oncogene-associated, multicolour reporter mouse model-the Red2Onco system-that allows differential tracing of mutant and wild-type cells in the same tissue. By applying this system to the small intestine, we show that oncogene-expressing mutant crypts alter the cellular organization of neighbouring wild-type crypts, thereby driving accelerated clonal drift. Crypts that express oncogenic KRAS or PI3K secrete BMP ligands that suppress local stem cell activity, while changes in PDGFR(lo)CD81(+) stromal cells induced by crypts with oncogenic PI3K alter the WNT signalling environment. Together, these results show how oncogene-driven paracrine remodelling creates a niche environment that is detrimental to the maintenance of wild-type tissue, promoting field transformation dominated by oncogenic clones.

【 授权许可】

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