Wnt11 patterns a myocardial electrical gradient through regulation of the L-type Ca2+ channel

Article

关键词: VOLTAGE-SENSITIVE DYES;    CARDIAC TISSUE;    CELL-CULTURES;    CONDUCTION;    GASTRULATION;    ACTIVATION;    MOVEMENTS;    DIFFERENTIATION;    MORPHOGENESIS;    MECHANISMS;   

DOI  :  10.1038/nature09249

来源: SCIE

【 摘 要 】

Electrical gradients are critical for many biological processes, including the normal function of excitable tissues, left-right patterning, organogenesis and wound healing(1-4). The fundamental mechanisms that regulate the establishment and maintenance of such electrical polarities are poorly understood. Here we identify a gradient of electrical coupling across the developing ventricular myocardium using high-speed optical mapping of transmembrane potentials and calcium concentrations in the zebrafish heart. We excluded a role for differences in cellular excitability, connexin localization, tissue geometry and mechanical inputs, but in contrast we were able to demonstrate that non-canonical Wnt11 signals are required for the genesis of this myocardial electrical gradient. Although the traditional planar cell polarity pathway is not involved, we obtained evidence that Wnt11 acts to set up this gradient of electrical coupling through effects on transmembrane Ca2+ conductance mediated by the L-type calcium channel. These data reveal a previously unrecognized role for Wnt/Ca2+ signalling in establishing an electrical gradient in the plane of the developing cardiac epithelium through modulation of ion-channel function. The regulation of cellular coupling through such mechanisms may be a general property of non-canonical Wnt signals.

【 授权许可】

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