A physical, genetic and functional sequence assembly of the barley genome

Article

关键词: MESSENGER-RNA DECAY;    MILDEW RESISTANCE LOCUS;    HORDEUM-VULGARE L.;    NONCODING RNAS;    EVOLUTION;    MAP;    EXPRESSION;    RICE;    ARABIDOPSIS;    REVEALS;   

DOI  :  10.1038/nature11543

来源: SCIE

【 摘 要 】

Barley (Hordeum vulgare L.) is among the world's earliest domesticated and most important crop plants. It is diploid with a large haploid genome of 5.1 gigabases (Gb). Here we present an integrated and ordered physical, genetic and functional sequence resource that describes the barley gene-space in a structured whole-genome context. We developed a physical map of 4.98 Gb, with more than 3.90 Gb anchored to a high-resolution genetic map. Projecting a deep whole-genome shotgun assembly, complementary DNA and deep RNA sequence data onto this framework supports 79,379 transcript clusters, including 26,159 'high-confidence' genes with homology support from other plant genomes. Abundant alternative splicing, premature termination codons and novel transcriptionally active regions suggest that post-transcriptional processing forms an important regulatory layer. Survey sequences from diverse accessions reveal a landscape of extensive single-nucleotide variation. Our data provide a platform for both genome-assisted research and enabling contemporary crop improvement.

【 授权许可】

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