Architecture of a channel-forming O-antigen polysaccharide ABC transporter

Article

关键词: CRYSTAL-STRUCTURES;    LIPOPOLYSACCHARIDE;    BINDING;    BIOSYNTHESIS;    PHENIX;    TOOLS;    MODEL;   

DOI  :  10.1038/nature25190

来源: SCIE

【 摘 要 】

O-antigens are cell surface polysaccharides of many Gram-negative pathogens that aid in escaping innate immune responses(1). A widespread O-antigen biosynthesis mechanism involves the synthesis of the lipid-anchored polymer on the cytosolic face of the inner membrane, followed by transport to the periplasmic side where it is ligated to the lipid A core to complete a lipopolysaccharide molecule(2). In this pathway, transport to the periplasm is mediated by an ATP-binding cassette (ABC) transporter, called Wzm-Wzt. Here we present the crystal structure of the Wzm-Wzt homologue from Aquifex aeolicus in an open conformation. The transporter forms a transmembrane channel that is sufficiently wide to accommodate a linear polysaccharide. Its nucleotide-binding domain and a periplasmic extension form 'gate helices' at the cytosolic and periplasmic membrane interfaces that probably serve as substrate entry and exit points. Site-directed mutagenesis of the gates impairs in vivo O-antigen secretion in the Escherichia coli prototype. Combined with a closed structure of the isolated nucleotide-binding domains, our structural and functional analyses suggest a processive O-antigen translocation mechanism, which stands in contrast to the classical alternating access mechanism of ABC transporters.

【 授权许可】

Free