Visualizing the mechanical activation of Src

Article

关键词: GREEN FLUORESCENT PROTEIN;    FOCAL ADHESIONS;    FAMILY KINASES;    LIVING CELLS;    V-SRC;    CYTOSKELETON;    DOMAIN;    SPECIFICITY;    P130(CAS);    MEMBRANE;   

DOI  :  10.1038/nature03469

来源: SCIE

【 摘 要 】

The mechanical environment crucially influences many cell functions(1). However, it remains largely mysterious how mechanical stimuli are transmitted into biochemical signals. Src is known to regulate the integrin-cytoskeleton interaction(2), which is essential for the transduction of mechanical stimuli(3-5). Using fluorescent resonance energy transfer (FRET), here we develop a genetically encoded Src reporter that enables the imaging and quantification of spatio-temporal activation of Src in live cells. We introduced a local mechanical stimulation to human umbilical vein endothelial cells (HUVECs) by applying laser-tweezer traction on fibronectin-coated beads adhering to the cells. Using the Src reporter, we observed a rapid distal Src activation and a slower directional wave propagation of Src activation along the plasma membrane. This wave propagated away from the stimulation site with a speed (mean +/- s.e.m.) of 18.1 +/- 1.7 nm s(-1). This force-induced directional and long-range activation of Src was abolished by the disruption of actin filaments or microtubules. Our reporter has thus made it possible to monitor mechanotransduction in live cells with spatio-temporal characterization. We find that the transmission of mechanically induced Src activation is a dynamic process that directs signals via the cytoskeleton to spatial destinations.

【 授权许可】

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