Transcriptome analysis of Populus × canadensis ‘Zhongliao1’ in response to low temperature stress

BMC Genomics

Research

Hua Jin¹  Chengchao Yang²  Yan Zhang²  Xiaoyu Li² 

[1] College of Environment and Bioresources, Dalian Minzu University, 116600, Dalian, China;Liaoning Provincial Institute of Poplar, 115213, Gaizhou, China;

关键词: Poplar;    Freeze–thaw injury;    Overwintering;    Low temperature stress;    Cold stress response;   

DOI  :  10.1186/s12864-023-09187-7

 received in 2022-10-31, accepted in 2023-02-14,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundLow temperatures are known to limit the growth and geographical distribution of poplars. Although some transcriptomic studies have been conducted to explore the response of poplar leaves to cold stress, only a few have comprehensively analyzed the effects of low temperature on the transcriptome of poplars and identified genes related to cold stress response and repair of freeze–thaw injury.ResultsWe exposed the Euramerican poplar Zhongliao1 to low temperatures; after stems were exposed to − 40℃, 4℃, and 20℃, the mixture of phloem and cambium was collected for transcriptome sequencing and bioinformatics analysis. A total of 29,060 genes were detected, including 28,739 known genes and 321 novel genes. Several differentially expressed genes (n = 36) were found to be involved in the Ca2+ signaling pathway, starch–sucrose metabolism pathway, abscisic acid signaling pathway, and DNA repair. They were functionally annotated; glucan endo-1,3-beta-glucosidase and UDP-glucuronosyltransferase genes, for instance, showed a close relationship with cold resistance. The expression of 11 differentially expressed genes was verified by qRT-PCR; RNA-Seq and qRT-PCR data were found to be consistent, which validated the robustness of our RNA-Seq findings. Finally, multiple sequence alignment and evolutionary analysis were performed, the results of which suggested a close association between several novel genes and cold resistance in Zhongliao1.ConclusionWe believe that the cold resistance and freeze–thaw injury repair genes identified in this study are of great significance for cold tolerance breeding.

【 授权许可】

CC BY   
© The Author(s) 2023

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