Comparative assessment of coronary physiology using transthoracic pulsed-wave Doppler and myocardial contrast echocardiography in rats

European Radiology Experimental

Original Article

Moritz Uhlig¹  Anna B. Roehl¹  Marc Hein¹  Sebastian Billig¹  Mare Mechelinck¹  David Schumacher²  Dieter Fuchs³  Rafael Kramann⁴ 

[1] Department of Anesthesiology, Faculty of Medicine, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany;Department of Anesthesiology, Faculty of Medicine, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany;Institute of Experimental Medicine and Systems Biology, Faculty of Medicine, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany;FUJIFILM VisualSonics, Inc., Joop Geesinkweg 140, 1114, Amsterdam, AB, The Netherlands;Institute of Experimental Medicine and Systems Biology, Faculty of Medicine, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany;Division of Nephrology and Clinical Immunology, Faculty of Medicine, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany;Department of Internal Medicine, Nephrology and Transplantation, Erasmus Medical, Doctor Molewaterplein 40, 3015, Rotterdam, GD, The Netherlands;

关键词: Adenosine;    Coronary vessels;    Dobutamine;    Echocardiography;    Rats;   

DOI  :  10.1186/s41747-022-00319-4

 received in 2022-08-04, accepted in 2022-12-28,  发布年份 2022

来源: Springer

【 摘 要 】

BackgroundCoronary physiology assessment in rodents by ultrasound is an excellent noninvasive and easy to perform technique, including pulsed-wave Doppler (PWD) and myocardial contrast echocardiography (MCE). Both techniques and the corresponding calculated parameters were investigated in this study at rest as well as their response to pharmacologically induced stress.MethodsLeft ventricular myocardial function was assessed in eight anaesthetised rats using transthoracic echocardiography. Coronary physiology was assessed by both PWD of the left coronary artery and MCE using a bolus method. Measurements were performed at rest and under stimulation with adenosine and dobutamine. Effects of stimulation on the calculated parameters were evaluated and rated by effect size (η2).ResultsChanges could be demonstrated by selected parameters of PWD and MCE. The clearest effect in PWD was found for diastolic peak velocity (η2 = 0.58). It increased from 528 ± 110 mm/s (mean ± standard deviation) at rest to 839 ± 342 mm/s (p = 0.001) with adenosine and 1093 ± 302 mm/s with dobutamine (p = 0.001). The most distinct effect from MCE was found for the normalised wash-in rate (η2 = 0.58). It increased from 1.95 ± 0.35% at rest to 3.87 ± 0.85% with adenosine (p = 0.001) and 3.72 ± 1.03% with dobutamine (p = 0.001).ConclusionInduced changes in coronary physiology by adenosine and dobutamine could successfully be monitored using MCE and PWD in anaesthetised rats. Due to the low invasiveness of the measurements, this protocol could be used for longitudinal animal studies.

【 授权许可】

CC BY   
© The Author(s) 2023

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