Prognosis-related molecular subtyping in head and neck squamous cell carcinoma patients based on glycolytic/cholesterogenic gene data

Cancer Cell International

Research

Jianfei Tang¹  Xiaohan Dai¹  Jia Jia¹  Yixuan Lu¹  Kaixin Su¹  Ousheng Liu¹  Tiao Luo¹  Zekun Zhou¹  Haixia Zhang² 

[1] Hunan Key Laboratory of Oral Health Research & Hunan 3D Printing Engineering Research Center of Oral Care & Hunan Clinical Research Center of Oral Major Diseases and Oral Health & Academician Workstation for Oral-maxilofacial and Regenerative Medicine & Xiangya Stomatological Hospital & Xiangya School of Stomatology, Central South University, 410008, Changsha, Hunan, China;The Oncology Department of Xiangya Second Hospital, Central South University, 410011, Changsha, Hunan, China;

关键词: Bioinformatics;    Metabolic classification;    Prognosis;    Head and neck squamous cell carcinoma;    Glycolysis;    Cholesterogenic;   

DOI  :  10.1186/s12935-023-02880-3

 received in 2022-07-29, accepted in 2023-02-19,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundHead and neck squamous cell carcinoma (HNSCC) remains an unmet medical challenge. Metabolic reprogramming is a hallmark of diverse cancers, including HNSCC.MethodsWe investigated the metabolic profile in HNSCC by using The Cancer Genome Atlas (TCGA) (n = 481) and Gene Expression Omnibus (GEO) (n = 97) databases. The metabolic stratification of HNSCC samples was identified by using unsupervised k-means clustering. We analyzed the correlations of the metabolic subtypes in HNSCC with featured genomic alterations and known HNSCC subtypes. We further validated the metabolism-related subtypes based on features of ENO1, PFKFB3, NSDHL and SQLE expression in HNSCC by Immunohistochemistry. In addition, genomic characteristics of tumor metabolism that varied among different cancer types were confirmed.ResultsBased on the median expression of coexpressed cholesterogenic and glycolytic genes, HNSCC subtypes were identified, including glycolytic, cholesterogenic, quiescent and mixed subtypes. The quiescent subtype was associated with the longest survival and was distributed in stage I and G1 HNSCC. Mutation analysis of HNSCC genes indicated that TP53 has the highest mutation frequency. The CDKN2A mutation frequency has the most significant differences amongst these four subtypes. There is good overlap between our metabolic subtypes and the HNSCC subtype.ConclusionThe four metabolic subtypes were successfully determined in HNSCC. Compared to the quiescent subtype, glycolytic, cholesterogenic and mixed subtypes had significantly worse outcome, which might offer guidelines for developing a novel treatment strategy for HNSCC.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 2

Fig. 1

Fig. 5

Fig. 4

Fig. 4

Fig. 1

40708_2023_184_Article_IEq4.gif

Fig. 4