Screening crucial lncRNAs and genes in osteoarthritis by integrated analysis

Advances in Rheumatology

Research

Tao Ma¹  Pengfei Wen¹  Jun Wang¹  Wei Song¹  Binfei Zhang¹  Tao Wang¹  Yumin Zhang¹ 

[1]Department of Joint Surgery, Honghui Hospital, Xi’an Jiaotong University, No.555, Youyi East Road Nanshaomen, 710054, Xi’an, Shaanxi, China

关键词: Osteoarthritis;    GEO;    lncRNA;    mRNA;    Bioinformatics;    Diagnosis;   

DOI  :  10.1186/s42358-023-00288-1

 received in 2022-07-13, accepted in 2023-02-18,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundOsteoarthritis (OA) is one of the most frequent chronic diseases with high morbidity worldwide, marked by degradation of the cartilage and bone, joint instability, stiffness, joint space stenosis and subchondral sclerosis. Due to the elusive mechanism of osteoarthritis (OA), we aimed to identify potential markers for OA and explore the molecular mechanisms underlying OA.MethodsExpression profiles data of OA were collected from the Gene Expression Omnibus database to identify differentially expressed mRNAs (DEmRNAs) and differentially expressed lncRNAs (DElncRNAs) in OA. Functional annotation and protein–protein interaction (PPI) networks were performed. Then, nearby DEmRNAs of DElncRNAs was obtained. Moreover, GO and KEGG pathway enrichment analysis of nearby DEmRNAs of DElncRNAs was performed. Finally, expression validation of selected mRNAs and lncRNAs was performed by quantitative reverse transcriptase-polymerase chain reaction.ResultsIn total, 2080 DEmRNAs and 664 DElncRNAs were determined in OA. PI3K-Akt signaling pathway, Endocytosis and Rap1 signaling pathway were significantly enriched KEGG pathways in OA. YWHAB, HSPA8, NEDD4L and SH3KBP1 were four hub proteins in PPI network. The AC093484.4/TRPV2 interact pair may be involved in the occurrence and development of OA.ConclusionOur study identified several DEmRNAs and DElncRNAs associated with OA. The molecular characters could provide more information for further study on OA.

【 授权许可】

CC BY   
© The Author(s) 2023

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