Dynamic interaction of injected liquid jet with skin layer interfaces revealed by microsecond imaging of optically cleared ex vivo skin tissue model

Journal of Biological Engineering

Research

Jakir Hossain Imran¹  Jung Kyung Kim²  Kee Sung Lee²  Abdul Mohizin² 

[1] Department of Mechanical Engineering, Graduate School, Kookmin University, 02707, Seoul, Republic of Korea;School of Mechanical Engineering, Kookmin University, 77 Jeongneung-Ro, Seongbuk-Gu, 02707, Seoul, Republic of Korea;

关键词: Needle-free jet injection;    Transdermal drug delivery;    Interfacial interaction;    Deep tissue imaging;    Near-infrared imaging;    Tissue clearing;    Skin tissue model;   

DOI  :  10.1186/s13036-023-00335-x

 received in 2022-12-09, accepted in 2023-02-21,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundNeedle-free jet injection (NFJI) systems enable a controlled and targeted delivery of drugs into skin tissue. However, a scarce understanding of their underlying mechanisms has been a major deterrent to the development of an efficient system. Primarily, the lack of a suitable visualization technique that could capture the dynamics of the injected fluid–tissue interaction with a microsecond range temporal resolution has emerged as a main limitation. A conventional needle-free injection system may inject the fluids within a few milliseconds and may need a temporal resolution in the microsecond range for obtaining the required images. However, the presently available imaging techniques for skin tissue visualization fail to achieve these required spatial and temporal resolutions. Previous studies on injected fluid–tissue interaction dynamics were conducted using in vitro media with a stiffness similar to that of skin tissue. However, these media are poor substitutes for real skin tissue, and the need for an imaging technique having ex vivo or in vivo imaging capability has been echoed in the previous reports.MethodsA near-infrared imaging technique that utilizes the optical absorption and fluorescence emission of indocyanine green dye, coupled with a tissue clearing technique, was developed for visualizing a NFJI in an ex vivo porcine skin tissue.ResultsThe optimal imaging conditions obtained by considering the optical properties of the developed system and mechanical properties of the cleared ex vivo samples are presented. Crucial information on the dynamic interaction of the injected liquid jet with the ex vivo skin tissue layers and their interfaces could be obtained.ConclusionsThe reported technique can be instrumental for understanding the injection mechanism and for the development of an efficient transdermal NFJI system as well.

【 授权许可】

CC BY   
© The Author(s) 2023

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