In vivo imaging of inflammatory response in cancer research

Inflammation and Regeneration

Review

Yoshinobu Konishi¹  Kenta Terai² 

[1]Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA

[2]Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto, Japan

关键词: Intravital microscopy;    Two-photon excitation microscope;    Tumor microenvironment;    Förster resonance energy transfer;    Optogenetics;   

DOI  :  10.1186/s41232-023-00261-x

 received in 2022-09-07, accepted in 2023-01-27,  发布年份 2023

来源: Springer

【 摘 要 】

Inflammation can contribute to the development and progression of cancer. The inflammatory responses in the tumor microenvironment are shaped by complex sequences of dynamic intercellular cross-talks among diverse types of cells, and recapitulation of these dynamic events in vitro has yet to be achieved. Today, intravital microscopy with two-photon excitation microscopes (2P-IVM) is the mainstay technique for observing intercellular cross-talks in situ, unraveling cellular and molecular mechanisms in the context of their spatiotemporal dynamics. In this review, we summarize the current state of 2P-IVM with fluorescent indicators of signal transduction to reveal the cross-talks between cancer cells and surrounding cells including both immune and non-immune cells. We also discuss the potential application of red-shifted indicators along with optogenetic tools to 2P-IVM. In an era of single-cell transcriptomics and data-driven research, 2P-IVM will remain a key advantage in delivering the missing spatiotemporal context in the field of cancer research.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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