Matrine alleviates depressive-like behaviors via modulating microbiota–gut–brain axis in CUMS-induced mice

Journal of Translational Medicine

Research

Aoqiang Li¹  Jingyi Li²  Guannan Wang²  Ying Sun²  Zhenbo Song²  Lihua Zheng²  Lei Liu²  Qifang Yang²  Yanxin Huang²  Ming Zhang³  Muqing Zhang⁴ 

[1] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China;National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, China;National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China;School of Molecular & Cellular Biology, University of Illinois Urbana Champaign, Urbana, IL, USA;

关键词: Matrine;    Depression;    Gut microbiota;    Metabolomics;    Microbiota–gut–brain axis;   

DOI  :  10.1186/s12967-023-03993-z

 received in 2022-11-20, accepted in 2023-02-15,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundThe realization of the “microbiota–gut–brain” axis plays a critical role in neuropsychiatric disorders, particularly depression, is advancing rapidly. Matrine is a natural bioactive compound, which has been found to possess potential antidepressant effect. However, the underlying mechanisms of regulation of the “microbiota–gut–brain” axis in the treatment of depression by oral matrine remain elusive.MethodsIts antidepressant effects were initially evaluated by behavioral tests and relative levels of monoamine neurotransmitters, and matrine has been observed to attenuate the depression-like behavior and increase neurotransmitter content in CUMS-induced mice. Subsequently, studies from the “gut” to “brain” were conducted, including detection of the composition of gut microbiota by 16S rRNA sequencing; the metabolomics detection of gut metabolites and the analysis of differential metabolic pathways; the assessment of relative levels of diamine oxidase, lipopolysaccharide, pro-inflammatory cytokines, and brain-derived neurotrophic factor (BDNF) by ELISA kits or immunofluorescence.ResultsMatrine could regulate the disturbance of gut microbiota and metabolites, restore intestinal permeability, and reduce intestinal inflammation, thereby reducing the levels of pro-inflammatory cytokines in peripheral blood circulation and brain regions, and ultimately increase the levels of BDNF in brain.ConclusionMatrine may ameliorate CUMS-induced depression in mice by modulating the “microbiota–gut–brain” axis.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 4

Fig. 3

12888_2023_4625_Article_IEq1.gif

Fig. 1