Hypochlorous acid inactivates oral pathogens and a SARS-CoV-2-surrogate

BMC Oral Health

Research

J. Christopher Fenno¹  Mariane Maffei Azuma²  Neville J. McDonald²  Rutuja Jadhav²  Hajime Sasaki²  Kento Tazawa³ 

[1] Department of Biologic and Materials Sciences & Prosthodontics, School of Dentistry, University of Michigan, 48109, Ann Arbor, MI, USA;Department of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, 1011 N University Ave, 48109, Ann Arbor, MI, USA;Department of Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, 1011 N University Ave, 48109, Ann Arbor, MI, USA;Division of Oral Health Sciences, Department of Pulp Biology and Endodontics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan;

关键词: Hypochlorous acid (HOCl);    Dental practice;    Airborne infection;    Oral pathogens;    SARS-CoV-2;    Disinfectant;    Mouthwash;    Minimum inhibitory volume ratio;   

DOI  :  10.1186/s12903-023-02820-7

 received in 2022-06-22, accepted in 2023-02-15,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundDroplets and aerosols produced during dental procedures are a risk factor for microbial and viral transmission. Unlike sodium hypochlorite, hypochlorous acid (HOCl) is nontoxic to tissues but still exhibits broad microbicidal effect. HOCl solution may be applicable as a supplement to water and/or mouthwash. This study aims to evaluate the effectiveness of HOCl solution on common human oral pathogens and a SARS-CoV-2 surrogate MHV A59 virus, considering the dental practice environment.MethodsHOCl was generated by electrolysis of 3% hydrochloric acid. The effect of HOCl on human oral pathogens, Fusobacterium nucleatum, Prevotella intermedia, Streptococcus intermedius, Parvimonas micra, and MHV A59 virus was studied from four perspectives: concentration; volume; presence of saliva; and storage. HOCl solution in different conditions was utilized in bactericidal and virucidal assays, and the minimum inhibitory volume ratio that is required to completely inhibit the pathogens was determined.ResultsIn the absence of saliva, the minimum inhibitory volume ratio of freshly prepared HOCl solution (45–60 ppm) was 4:1 for bacterial suspensions and 6:1 for viral suspensions. The presence of saliva increased the minimum inhibitory volume ratio to 8:1 and 7:1 for bacteria and viruses, respectively. Applying a higher concentration of HOCl solution (220 or 330 ppm) did not lead to a significant decrease in the minimum inhibitory volume ratio against S. intermedius and P. micra. The minimum inhibitory volume ratio increases in applications of HOCl solution via the dental unit water line. One week of storage of HOCl solution degraded HOCl and increased the minimum growth inhibition volume ratio.ConclusionsHOCl solution (45–60 ppm) is still effective against oral pathogens and SAR-CoV-2 surrogate viruses even in the presence of saliva and after passing through the dental unit water line. This study indicates that the HOCl solution can be used as therapeutic water or mouthwash and may ultimately reduce the risk of airborne infection in dental practice.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 8