Analysis of the Genetic Stability of Insect and Herbicide Resistance Genes in Transgenic Rice Lines: A Laboratory and Field Experiment

Rice

Research

Xiaosong Peng¹  Xiaoli Lin¹  Lu Qin¹  Bai Li¹  Changlan Zhu¹  Chunlei Wang¹  Linjuan Ouyang¹  Dahu Zhou¹  Haohua He¹  Yicong Cai¹  Zhongkai Chen²  Yue Sun³  Huizhen Chen⁴ 

[1] Key Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural University, Nanchang, Jiangxi, China;Key Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural University, Nanchang, Jiangxi, China;College of Agronomy, Hunan Agricultural University, Changsha, Hunan, China;Key Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural University, Nanchang, Jiangxi, China;Hainan Yazhou Bay Seed Laboratory, Sanya, Hainan, China;Key Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural University, Nanchang, Jiangxi, China;Pingxiang Center for Agricultural Sciences and Technology Research, Pingxiang, Jiangxi, China;

关键词: Bacillus thuringiensis;    Genetic stability;    Transgenic rice;    Insect resistance;    Herbicide resistance;   

DOI  :  10.1186/s12284-023-00624-5

 received in 2022-09-15, accepted in 2023-02-06,  发布年份 2023

来源: Springer

【 摘 要 】

A lack of stability in the expression of Bacillus thuringiensis genes (CRY) and the dialaninophosphate resistance gene (BAR) in transgenic rice plants can lead to the loss of important characters. The genetic stability of transgenic expression in high-generation lines is thus critically important for ensuring the success of molecular breeding efforts. Here, we studied the genetic stability of resistance to insect pests and herbicides in transgenic rice lines at the molecular and phenotypic levels in a pesticide-free environment. Southern blot analysis, real-time polymerase chain reaction, and enzyme-linked immunosorbent assays revealed high stability in the copy numbers and expression levels of CRY1C, CRY2A, and BAR in transgenic lines across different generations, and gene expression levels were highly correlated with protein expression levels. The insecticide resistance of the transgenic rice lines was high. The larval mortality of Chilo suppressalis was 50.25% to 68.36% higher in transgenic lines than in non-transgenic control lines. Percent dead hearts and percent white spikelets were 16.66% to 22.15% and 27.07% to 33.47% lower in transgenic lines than in non-transgenic control lines, respectively. The herbicide resistance of the transgenic rice lines was also high. The bud length and root length ranged were 2.53 cm to 4.20 cm and 0.28 cm to 0.73 cm higher in transgenic lines than in non-transgenic control lines in the budding stage, respectively. Following application of the herbicide Basta, the chlorophyll content of the transgenic lines began to recover 2 d later in the seedling and tillering stages and 3 d later in the booting and heading stages, by contrast, the chlorophyll content of the non-transgenic lines did not recover and continued to decrease. These findings revealed high genetic stability of the resistance to insect pests and herbicides across several generations of transgenic rice regardless of the genetic background.

【 授权许可】

CC BY   
© The Author(s) 2023

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