The therapeutic effect of larval saliva and hemolymph of Lucilia sericata on the treatment of Leishmania major lesion in BALB/c mice946

Parasites & Vectors

Research

Ali Khamesipour¹  Mahmood Bakhtiyari²  Kamran Akbarzadeh³  Javad Rafinejad³  Amir Ahmad Akhavan³  Sara Rahimi⁴  Reza Ahmadkhaniha⁵ 

[1] Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran;Department of Community Medicine and Epidemiology, School of Medicine Non-Communicable Diseases Research Center Alborz University of Medical Sciences, Karaj, Iran;Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;Medicinal Plants Research Center, Maragheh University of Medical Sciences, Maragheh, Iran;Pharmaceutical Chemistry, Department of Human Ecology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;

关键词: Leishmanial activity;    Lucilia sericata;    Leishmania major;    Cutaneous leishmaniasis;    Glucantime;    Natural compound;   

DOI  :  10.1186/s13071-023-05660-0

 received in 2023-01-01, accepted in 2023-01-10,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundTreatment of cutaneous leishmaniasis (CL) remains a major challenge for the public health and medical community. It has been claimed that natural compounds derived from fly larvae have anti-leishmania properties against some species of Leishmania. The present study aimed at assessing the in vitro effects of larval products of Lucilia sericata against the promastigote and intracellular amastigote forms of Leishmania major. Also, the therapeutic effect of larval products on lesions induced by L. major infection was evaluated in BALB/c mice models.MethodsParasite specimens and macrophage cells were exposed to varying concentrations of larval products for 24–120 h. Lesion progression and parasite load were investigated in the models to assess the therapeutic effects of the products.ResultsThe larval products displayed more potent cytotoxicity against L. major promastigotes. The IC50 values for larval saliva and hemolymph were 100.6 and 37.96 ug/ml, respectively. The IC50 of glucantime was 9.480 ug/ml. Also, the saliva and hemolymph of L. sericata exhibited higher cytotoxicity against the promastigotes of L. major but were less toxic to the macrophage cells. Treatment with leishmanicidal agents derived from larvae of L. sericata decreased the infection rate and the number of amastigotes per infected host cell at all concentrations. Lesion size was significantly (F(7, 38) = 8.54, P < 0.0001) smaller in the treated mice compared with the untreated control group. The average parasite burden in the treated mice groups (1.81 ± 0.74, 1.03 ± 0.45 and 3.37 ± 0.41) was similar to the group treated with a daily injection of glucantime (1.77 ± 0.99) and significantly lower (F(7, 16) = 66.39, P < 0.0001) than in the untreated control group (6.72 ± 2.37).ConclusionsThe results suggest that the larval products of L. sericata were effective against L. major parasites both in vivo and in vitro. However, more clinical trial studies are recommended to evaluate the effects of these larval products on human subjects.Graphical Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

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