Longitudinal study of the short- and long-term effects of hospitalisation and oral trimethoprim-sulfadiazine administration on the equine faecal microbiome and resistome

Microbiome

Research

Roosmarijn E. C. Luiken¹  Jaap A. Wagenaar²  Aldert L. Zomer²  Marianne M. Sloet van Oldruitenborgh-Oosterbaan³  Mathijs J. P. Theelen⁴  David A. van Doorn⁵  John W. A. Rossen⁶  Femke J. W. C. Schaafstra⁷ 

[1] Department of Biomolecular Health Sciences (Infectious Diseases and Immunology), Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584, Utrecht, CL, the Netherlands;Department of Biomolecular Health Sciences (Infectious Diseases and Immunology), Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584, Utrecht, CL, the Netherlands;WHO Collaborating Centre for Reference and Research on Campylobacter and Antimicrobial Resistance from a One Health Perspective/OIE Reference Laboratory for Campylobacteriosis, Yalelaan 1, 3584, Utrecht, CL, the Netherlands;Department of Clinical Sciences (Equine Sciences), Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, 3584, Utrecht, CM, the Netherlands;Department of Clinical Sciences (Equine Sciences), Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, 3584, Utrecht, CM, the Netherlands;Department of Biomolecular Health Sciences (Infectious Diseases and Immunology), Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584, Utrecht, CL, the Netherlands;Department of Clinical Sciences (Equine Sciences), Faculty of Veterinary Medicine, Utrecht University, Yalelaan 112, 3584, Utrecht, CM, the Netherlands;Department of Population Health Sciences (Farm Animal Health), Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584, Utrecht, CL, the Netherlands;Department of Medical Microbiology and Infection Prevention, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9713, Groningen, GZ, the Netherlands;Department of Pathology, University of Utah School of Medicine, 15 North Medical Drive East, Ste #1100, 84112, Salt Lake City, Utah, USA;HAS University of Applied Sciences, Onderwijsboulevard 221, 5223, ‘s-Hertogenbosch, DE, the Netherlands;Department of Population Health Sciences (Farm Animal Health), Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584, Utrecht, CL, the Netherlands;

关键词: Microbiota;    Horse;    Antimicrobial resistance;    Shotgun metagenomic sequencing;    Antimicrobial resistance genes;    sul2;    tetQ;    ant6-1a;    Aph(3”)-lb;    lnuC;   

DOI  :  10.1186/s40168-023-01465-6

 received in 2022-06-06, accepted in 2023-01-10,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundHospitalisation and antimicrobial treatment are common in horses and significantly impact the intestinal microbiota. Antimicrobial treatment might also increase levels of resistant bacteria in faeces, which could spread to other ecological compartments, such as the environment, other animals and humans. In this study, we aimed to characterise the short- and long-term effects of transportation, hospitalisation and trimethoprim-sulfadiazine (TMS) administration on the faecal microbiota and resistome of healthy equids.MethodsIn a longitudinal experimental study design, in which the ponies served as their own control, faecal samples were collected from six healthy Welsh ponies at the farm (D0–D13-1), immediately following transportation to the hospital (D13-2), during 7 days of hospitalisation without treatment (D14–D21), during 5 days of oral TMS treatment (D22–D26) and after discharge from the hospital up to 6 months later (D27–D211). After DNA extraction, 16S rRNA gene sequencing was performed on all samples. For resistome analysis, shotgun metagenomic sequencing was performed on selected samples.ResultsHospitalisation without antimicrobial treatment did not significantly affect microbiota composition. Oral TMS treatment reduced alpha-diversity significantly. Kiritimatiellaeota, Fibrobacteres and Verrucomicrobia significantly decreased in relative abundance, whereas Firmicutes increased. The faecal microbiota composition gradually recovered after discontinuation of TMS treatment and discharge from the hospital and, after 2 weeks, was more similar to pre-treatment composition than to composition during TMS treatment. Six months later, however, microbiota composition still differed significantly from that at the start of the study and Spirochaetes and Verrucomicrobia were less abundant. TMS administration led to a significant (up to 32-fold) and rapid increase in the relative abundance of resistance genes sul2, tetQ, ant6-1a, and aph(3”)-lb. lnuC significantly decreased directly after treatment. Resistance genes sul2 (15-fold) and tetQ (six-fold) remained significantly increased 6 months later.ConclusionsOral treatment with TMS has a rapid and long-lasting effect on faecal microbiota composition and resistome, making the equine hindgut a reservoir and potential source of resistant bacteria posing a risk to animal and human health through transmission. These findings support the judicious use of antimicrobials to minimise long-term faecal presence, excretion and the spread of antimicrobial resistance in the environment.-NEQ1286BccpFJdhcRCuNyVideo Abstract

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

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