White-light crosslinkable milk protein bioadhesive with ultrafast gelation for first-aid wound treatment

Biomaterials Research

Research Article

Tanchen Ren¹  Xiaoqian Hong¹  Xuhao Zhou¹  Di Ye²  Daxi Ren³  Qinchao Zhu³  Teresa G. Valencak³  Kang Yu⁴  Yanan Zhang⁵  Liwen Zhang⁶  Wangbei Cao⁶  Houwei Zheng⁶  Yang Zhu⁶  Chengchen Guo⁷  Ziyang Sun⁷  Yajun Zhang⁸  Ying Xiao⁸ 

[1] Department of Cardiology, Cardiovascular Key Laboratory of Zhejiang Province, Second Affiliated Hospital, School of Medicine, Zhejiang University, 310027, Hangzhou, China;Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, 310058, Hangzhou, China;Institute of Dairy Science, College of Animal Sciences, Zhejiang University, 310058, Hangzhou, China;Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province, School of Mechanical Engineering, Zhejiang University, 310027, Hangzhou, China;Key Laboratory of Animal Virology of Ministry of Agriculture, Center for Veterinary Sciences, Zhejiang University, 310058, Hangzhou, China;MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, 310027, Hangzhou, China;School of Engineering, Westlake University, 310023, Hangzhou, Zhejiang, China;Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, 310020, Hangzhou, Zhejiang, China;

关键词: Casein;    photocrosslink;    Bioadhesive;    Hemostasis;    Wound healing;   

DOI  :  10.1186/s40824-023-00346-1

 received in 2022-11-15, accepted in 2023-01-25,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundPost-traumatic massive hemorrhage demands immediately available first-aid supplies with reduced operation time and good surgical compliance. In-situ crosslinking gels that are flexibly adapting to the wound shape have a promising potential, but it is still hard to achieve fast gelation, on-demand adhesion, and wide feasibility at the same time.MethodsA white-light crosslinkable natural milk-derived casein hydrogel bioadhesive is presented for the first time. Benefiting from abundant tyrosine residues, casein hydrogel bioadhesive was synthesized by forming di-tyrosine bonds under white light with a ruthenium-based catalyst. We firstly optimized the concentration of proteins and initiators to achieve faster gelation and higher mechanical strength. Then, we examined the degradation, cytotoxicity, tissue adhesion, hemostasis, and wound healing ability of the casein hydrogels to study their potential to be used as bioadhesives.ResultRapid gelation of casein hydrogel is initiated with an outdoor flashlight, a cellphone flashlight, or an endoscopy lamp, which facilitates its usage during first-aid and minimally invasive operations. The rapid gelation enables 3D printing of the casein hydrogel and excellent hemostasis even during liver hemorrhage due to section injury. The covalent binding between casein and tissue enables robust adhesion which can withstand more than 180 mmHg blood pressure. Moreover, the casein-based hydrogel can facilitate post-traumatic wound healing caused by trauma due to its biocompatibility.ConclusionCasein-based bioadhesives developed in this study pave a way for broad and practical application in emergency wound management.

【 授权许可】

CC BY   
© The Author(s) 2023

【 图 表 】

Fig. 13

Fig. 2

Fig. 1

Fig. 2

Fig. 1

Fig. 2